Question 1

Which of the following is/are used to detect chlamydiae in tissues and cell scrapings?

Accepted Answer

A)  PCR methods.
B)  Microscopy of Giemsa stained samples.
C)  Immunofluorescence.
D)  All of the choices.E) C) and D)
F) A) and D)

Question 2

Which of the following can take weeks to produce obvious growth on routine culture media?

Accepted Answer

A)  mycoplasmas
B)  rickettsias
C)  chlamydiae
D)  protozoaE) All of the above
F) B) and C) A

Question 3

An elevated IgM titer typically indicates an active infection while an elevated IgG titer does not distinguish between active and past infections.

Accepted Answer

A) True 
 B)False

Question 4

________ can also be used to differentiate strains of microorganisms that differ in the antigenic composition of a structure or product.

Accepted Answer

The answer of ________ can also be used to differentiate...

Question 5

The indirect immunosorbent assay detects antibodies rather than antigens.

Accepted Answer

A) True 
 B)False

Question 6

Which of the following is not a guideline on selection,collection,and handling of clinical specimens?

Accepted Answer

A)  A small quantity of specimen should be obtained.
B)  The specimen selected should be representative of the infectious disease process.
C)  The specimen should be forwarded promptly to the clinical laboratory.
D)  Attention must be given to avoid contamination of the specimen.E) A) and B)
F) A) and C)

Question 7

Which of the following uses a radioisotope?

Accepted Answer

A)  RIA
B)  ELISA
C)  API 20E
D)  all of theseE) B) and D)
F) B) and C)

Question 8

Indirect immunofluorescence involves fixing the specimen onto a slide,adding fluorochrome-labeled antibodies and then incubating before examining on the microscope.

Accepted Answer

A) True 
 B)False

Question 9

Some viruses alter the plasma membrane of infected cells in such a way as to cause red blood cells to tightly adhere to the infected cells.This is called ___________.

Accepted Answer

The answer of Some viruses alter the plasma membrane of...

Question 10

The principle of plasmid fingerprinting is that microbial isolates of the same strain contain the same plasmids.

Accepted Answer

A) True 
 B)False

Question 11

Viral replication in cell cultures can be detected by

Accepted Answer

A)  the observation of cytopathic effects.
B)  hemadsorption.
C)  gram stain.
D)  the observation of cytopathic effects and hemadsorption.E) None of the above
F) B) and C)

Question 12

The most common method used by clinical microbiology labs for identifying bacteria are

Accepted Answer

A)  biochemical.
B)  morphological.
C)  PCR.
D)  DNA fingerprinting.E) B) and C)
F) A) and D) A

Question 13

Which of the following tests can be used to distinguish Escherichia from Enterobacter?

Accepted Answer

A)  IMVIC
B)  ONPG test
C)  citrate utilization
D)  starch hydrolysisE) C) and D)
F) A) and B)

Question 14

The Gram stain is used for bacteria that have cell walls while the acid-fast stain is used primarily for wall-less bacteria.

Accepted Answer

A) True 
 B)False

Question 15

A clinical specimen represents a portion or quantity of human material for testing,examination,or study in order to determine the presence or absence of specific microorganisms.

Accepted Answer

A) True 
 B)False  True

Question 16

Which of the following uses red blood cells?

Accepted Answer

A)  hemagglutination inhibition
B)  MIC
C)  ELISA
D)  RIAE) B) and D)
F) A) and C)

Question 17

Restriction endonucleases are a class of enzymes produced by prokaryotes.

Accepted Answer

A) True 
 B)False

Question 18

The major focus of the clinical microbiologist is to rapidly isolate and identify microorganisms from clinical specimens.

Accepted Answer

A) True 
 B)False

Question 19

The purpose of the clinical microbiology laboratory is to maintain cultures of microorganisms.

Accepted Answer

A) True 
 B)False

Question 20

Which of the following is routinely used to detect spirochetes in skin lesions in early syphilis?

Accepted Answer

A)  bright-field microscopy
B)  phase-contrast microscopy
C)  dark-field microscopy
D)  DNA fingerprintingE) A) and C)
F) None of the above

Exam 36: Clinical Microbiology and Immunology

Which of the following is/are used to detect chlamydiae in tissues and cell scrapings?

Correct AnswerverifiedShow Answer

Which of the following can take weeks to produce obvious growth on routine culture media?

Correct AnswerverifiedA

An elevated IgM titer typically indicates an active infection while an elevated IgG titer does not distinguish between active and past infections.

Correct AnswerverifiedShow Answer

________ can also be used to differentiate strains of microorganisms that differ in the antigenic composition of a structure or product.

Correct AnswerverifiedShow Answer

The indirect immunosorbent assay detects antibodies rather than antigens.

Correct AnswerverifiedShow Answer

Which of the following is not a guideline on selection,collection,and handling of clinical specimens?

Correct AnswerverifiedShow Answer

Which of the following uses a radioisotope?

Correct AnswerverifiedShow Answer

Indirect immunofluorescence involves fixing the specimen onto a slide,adding fluorochrome-labeled antibodies and then incubating before examining on the microscope.

Correct AnswerverifiedShow Answer

Some viruses alter the plasma membrane of infected cells in such a way as to cause red blood cells to tightly adhere to the infected cells.This is called ___________.

Correct AnswerverifiedShow Answer

The principle of plasmid fingerprinting is that microbial isolates of the same strain contain the same plasmids.

Correct AnswerverifiedShow Answer

Viral replication in cell cultures can be detected by

Correct AnswerverifiedShow Answer

The most common method used by clinical microbiology labs for identifying bacteria are

Correct AnswerverifiedA

Which of the following tests can be used to distinguish Escherichia from Enterobacter?

Correct AnswerverifiedShow Answer

The Gram stain is used for bacteria that have cell walls while the acid-fast stain is used primarily for wall-less bacteria.

Correct AnswerverifiedShow Answer

A clinical specimen represents a portion or quantity of human material for testing,examination,or study in order to determine the presence or absence of specific microorganisms.

Correct AnswerverifiedTrue

Which of the following uses red blood cells?

Correct AnswerverifiedShow Answer

Restriction endonucleases are a class of enzymes produced by prokaryotes.

Correct AnswerverifiedShow Answer

The major focus of the clinical microbiologist is to rapidly isolate and identify microorganisms from clinical specimens.

Correct AnswerverifiedShow Answer

The purpose of the clinical microbiology laboratory is to maintain cultures of microorganisms.

Correct AnswerverifiedShow Answer

Which of the following is routinely used to detect spirochetes in skin lesions in early syphilis?

Correct AnswerverifiedShow Answer

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